Thermo Fisher Scientific PCAF Monoclonal Antibody (5E11)
Thermo Fisher Scientific의 PCAF Monoclonal Antibody (5E11)는 인간 PCAF 단백질을 인식하는 Mouse IgG2a 형 항체로, WB, ICC/IF, ELISA에 적합합니다. Affinity chromatography로 정제되었으며, PBS buffer에 보존되어 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.03 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E11 |
| Immunogen | PCAF (AAH60823, 353–452 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
LEEEVYSQNS PIWDQDFLSA SSRTSQLGIQ TVINPPPVAG TISYNSTSSS LEQPNAGSSS PACKASSGLE ANPGEKRKMT DSHVLEEAKK PRVMGDIPME
Target Information
PCAF (histone acetyltransferase KAT2B) functions as a histone acetyltransferase (HAT) to promote transcriptional activation. It has significant histone acetyltransferase activity with core histones (H3 and H4) and nucleosome core particles.
PCAF also acetylates non-histone proteins such as ACLY, PLK4, and TBX5. It inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A.
Additionally, PCAF acts as a circadian transcriptional coactivator enhancing the activity of NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. It is involved in heart and limb development by mediating acetylation of TBX5 and regulating its nucleocytoplasmic shuttling.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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