Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), PE
HSF1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rat 모노클로날 항체(10H8), PE 형광 표지형. WB, IHC, ICC, ELISA 등 다양한 응용 가능. 565/576 nm 형광 특성, Protein G 정제, 4°C 보관. 연구용 전용 제품.
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Thermo Fisher Scientific HSF1 Monoclonal Antibody (10H8), PE
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Gel Shift (GS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Guinea pig, Hamster, Human, Mouse, Non-human primate, Rabbit, Rat |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H8 |
| Immunogen | Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 378–395 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931584 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45130 was sufficient for detection of HSF1 in 20 µg of heat-shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.
Detects approximately 85 kDa (unstressed cell lysates) and approximately 95 kDa (heat-shocked cell lysates).
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. The transcriptional activator HSF (Heat Shock Factor) binds to heat shock response elements (HSEs), which are conserved across species.
Human cells express two HSFs, HSF1 and HSF2, sharing 38% sequence identity.
- HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents.
- HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Under stress, HSF1 becomes a nuclear-localized trimer that binds DNA, and phosphorylation is required for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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