Thermo Fisher Scientific NLK Monoclonal Antibody (2B11)
NLK 단백질을 표적하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 2B11). Western blot, ELISA, PLA에 적합하며, 비결합형 액상 형태로 제공. PBS buffer에 보관하며, 세포 신호전달 및 Wnt 경로 연구용으로 이상적.
- 카탈로그번호
- H00051701-M02
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific NLK Monoclonal Antibody (2B11)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 10 µg/mL |
| in situ PLA | 1:1200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B11 |
| Immunogen | NLK (AAH64663, 416–515 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein:
DEGRLRYHTC MCKCCFSTST GRVYTSDFEP VTNPKFDDTF EKNLSSVRQV KEIIHQFILE QQKGNRVPLC INPQSAAFKS FISSTVAQPS EMPPSPLVWETarget Information
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. It acts as a positive effector of the non-canonical Wnt signaling pathway downstream of WNT5A, MAP3K7/TAK1, and HIPK2. Activation leads to phosphorylation of the histone methyltransferase SETDB1, forming a complex that interacts with PPARG and silences transcription of PPARG target genes, thereby inhibiting adipogenesis and promoting osteoblastogenesis in mesenchymal stem cells.
NLK negatively regulates:
- Canonical Wnt/β-catenin signaling by phosphorylating TCF7L2/TCF4 and LEF1.
- Notch signaling by phosphorylating NOTCH1.
- MYB family transcription factors.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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