Thermo Fisher Scientific HSF1 Monoclonal Antibody (4B4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg/mL

-

Gel Shift (GS)

1mg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

4B4

Immunogen

Recombinant mouse HSF1 protein (aa1-503) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSF1,uniProtId:P38532-1,ncbiNodeId:10090,antigenRange:1-503,antigenLength:525,antigenImageFileName:MA5-14622_HSF1_P38532-1_Rat.svg,antigenImageFileNamePDP:MA5-14622_HSF1_P38532-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981502

Product Specific Information

MA5-14622 targets Heat Shock Factor 1 in GS, IF, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-14622 immunogen is recombinant mouse HSF1 protein (aa1-503).

Target Information

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.