Thermo Fisher Scientific MAP2K3 Monoclonal Antibody (OTI5C7), TrueMAB
MAP2K3 단백질을 인식하는 고특이성 Mouse Monoclonal Antibody로, WB, IHC, ICC/IF에 적합. Human 및 Dog 반응성. Lyophilized 형태로 제공되며, 재구성 후 다양한 실험에 활용 가능. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full length human recombinant MAP2K3 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase involved in cellular signal transduction and necessary for glucose transporter expression. It activates MAPK14/p38-MAPK and can be stimulated by insulin. Activation of RAS oncogene leads to accumulation of active MAP2K3, resulting in constitutive MAPK14 activation and oncogenic transformation. Inhibition of MAP2K3 is implicated in Yersina pseudotuberculosis pathogenesis. Multiple alternatively spliced transcript variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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