Thermo Fisher Scientific ZAP70 Polyclonal Antibody
Rabbit polyclonal antibody targeting human ZAP-70 protein kinase. Validated for Western blot and immunoprecipitation. Supplied as a liquid, unconjugated form at 1 mg/mL. Recommended storage at 4°C, shipped on wet ice. For research use only.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 564 and 614 of Human zeta-chain (TCR) associated protein kinase 70kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase that belongs to the Syk family of proteins. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains.
Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70 function in lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates this signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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