Thermo Fisher Scientific SQSTM1 Polyclonal Antibody
SQSTM1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow, IP 등 다양한 응용에 사용 가능. 인산화 및 NF-κB 신호전달 연구에 적합하며, 고순도의 항원 친화 정제 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:200 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Human, Insect, Mouse, Rat |
| Published Species | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 157–251 of Human SQSTM1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.39 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544723 |
Product Specific Information
- Recommended positive controls: A549, HepG2, HepG2 (3µM Thapsigargin, 24 hr), Huh7, Huh7 (3µM Thapsigargin, 12 hr), NIH-3T3, JC, BCL-1, PC-12, Rat2, HeLa.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the NF-κB signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-κB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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