Thermo Fisher Scientific SQSTM1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:200

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Bovine, Human, Insect, Mouse, Rat

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 157 and 251 of Human SQSTM1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SQSTM1,uniProtId:Q13501-1,ncbiNodeId:9606,antigenRange:157-251,antigenLength:440,antigenImageFileName:PA5-27247_SQSTM1_Q13501-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27247_SQSTM1_Q13501-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544723

Product Specific Information

Recommended positive controls: A549, HepG2, HepG2 (3µM Thapsigargin treatment for 24 hr), Huh7, Huh7 ( 3µM Thapsigargin, 12hr), NIH-3T3, JC, BCL-1, PC-12, Rat2, HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.