Thermo Fisher Scientific STIM1 Recombinant Rabbit Monoclonal Antibody (10N9E7)
Recombinant rabbit monoclonal antibody targeting human STIM1 for WB, IHC, ELISA, and IP applications. High specificity and affinity purified. Supplied as liquid form with 0.95 mg/mL concentration, suitable for calcium signaling research.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10N9E7 |
| Immunogen | Recombinant fusion protein containing amino acids 530–685 of human STIM1 (NP_003147.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.95 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093019 |
Product Specific Information
Immunogen sequence:
RQRVAPKPPQ MSRAADEALN AMTSNGSHRL IEGVHPGSLV EKLPDSPALA KKALLALNHG LDKAHSLMEL SPSAPPGGSP HLDSSRSHSP SSPDPDTPSP VGDSRALQAS RNTRIPHLAG KKAVAEEDNG SIGEETDSSP GRKKFPLKIF KKPLKK
Target Information
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Upon decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. The precise mechanism of STIM1–Orai1 interaction remains under investigation. STIM1 also associates with growing ends of microtubules and participates in endoplasmic reticulum tubule extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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