Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), Super Bright 702, eBioscience
마우스 CD90.2(Thy-1.2)를 인식하는 53-2.1 클론 단클론항체로, Super Bright 702 형광체가 결합된 Flow Cytometry용 항체입니다. T세포 및 뉴런 표지에 적합하며, 405 nm 레이저로 여기되어 702 nm에서 방출됩니다. 4°C 암소 보관 권장.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 7 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-2.1 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717157 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 615
- eFluor 450
- eFluor 506
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 555
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 760
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by mouse thymocytes, mature T cells, and neurons in CD90.2-expressing strains (e.g., BALB/c, CBA, C3H, C57BL/6, C58, SJL). Strains expressing CD90.1 (e.g., PL, AKR) do not stain with this antibody. CD90 plays a role in adhesion regulation and T-cell signal transduction.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Tested on mouse splenocytes; effective at ≤0.25 µg/test (100 µL final volume). Optimize antibody titration for best performance.
Super Bright 702 Dye:
Excitable with 405 nm violet laser, emits at 702 nm. Recommended filter: 710/50 bandpass. Ensure instrument compatibility.
Usage Recommendations:
- When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize polymer interactions.
- Protect from light due to photo-induced oxidation sensitivity.
- For fixation: use IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation: 405 nm
Emission: 702 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD90.2 (Thy-1) is a GPI-linked glycoprotein member of the immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. CD90.2 is an 18 kDa plasma membrane antigen found in hematopoietic cells and neurons, highly expressed in connective tissue, fibroblast, stromal, and tumor endothelial cell lines. In mice, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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