Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9)
인간 AKT1 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, Flow, ELISA, IP 등 다양한 응용에 사용 가능. 비인산화 및 인산화 형태 모두 검출 가능. Protein A로 정제된 액상 형태, -20°C 보관 권장.
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- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9)
Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (IHC) | 20 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14E5.A2.B2.H9 |
| Immunogen | Synthetic peptide corresponding to internal residues of human AKT1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Conjugated Formats
- APC
- Biotin
- FITC
- PE
- Custom conjugation available upon request
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-AKT1 antibody is directed against human AKT1 and detects both unphosphorylated and phosphorylated forms of the protein. Cross-reactivity with AKT1 from other species has not been determined, but the immunogen sequence shows 85% identity to mouse and 92% identity to rat, suggesting expected cross-reactivity.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
AKT1 is activated by insulin, PI3K, IGF1, and other growth and survival factors. It promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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