Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9)

Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• APC
• Biotin
• FITC
• PE
• Custom conjugation available upon request

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Anti-AKT1 antibody is directed against human AKT1 and detects both unphosphorylated and phosphorylated forms of the protein. Cross-reactivity with AKT1 from other species has not been determined, but the immunogen sequence shows 85% identity to mouse and 92% identity to rat, suggesting expected cross-reactivity.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells.
AKT1 is activated by insulin, PI3K, IGF1, and other growth and survival factors. It promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.