Thermo Fisher Scientific HIF3A Monoclonal Antibody (OTI2D2), TrueMAB
HIF3A 단백질을 인식하는 Mouse monoclonal antibody로 Western blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, 동결건조 형태로 제공. 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 355–669 of human HIF3A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cell growth and viability are affected by oxygen deprivation (hypoxia). Hypoxia-inducible factors (HIFs), including HIF-1α, HIF-1β (Arnt 1), EPAS-1 (HIF-2α), and HIF-3α, regulate glycolysis, erythropoiesis, and angiogenesis to restore oxygen homeostasis.
HIFs belong to the Per-Arnt-Sim (PAS) domain transcription factor family. Under hypoxic conditions, HIF-1α is upregulated and forms a heterodimer with Arnt 1, creating the HIF-1 complex. This complex binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, activating transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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