Thermo Fisher Scientific EEF1B2 Monoclonal Antibody (3D12)
EEF1B2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot과 ELISA에 사용 가능. Human 시료 반응성 확인. PBS 용액 형태로 제공되며, 보존제 없음. -20°C 보관, 동결/해동 반복 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D12 |
| Immunogen | EEF1B2 (AAH00211, 1–225 a.a.) full-length recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein sequence:
MGFGDLKSPA GLQVLNDYLA DKSYIEGYVP SQADVAVFEA VSSPPPADLC HALRWYNHIK SYEKEKASLP GVKKALGKYG PADVEDTTGS GATDSKDDDD IDLFGSDDEE ESEEAKRLRE ERLAQYESKK AKKPALVAKS SILLDVKPWD DETDMAKLEE CVRSIQADGL VWGSSKLVPV GYGIKKLQIQ CVVEDDKVGA DMLEEQITAF EDYVQSMDVA AFNKI
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for delivering aminoacyl-tRNAs to the ribosome. The EF-1 complex consists of four subunits: EF-1α, EF-1β, EF-1δ, and EF-1γ.
EF-1β is a 225 amino acid protein belonging to the EF-1β/EF-1δ family and contains a GST C-terminal domain. EF-1β and EF-1δ stimulate the exchange of GDP bound to EF-1α for GTP. Phosphorylation of EF-1β affects the GDP/GTP exchange rate. Alternative splicing of the EF-1β gene results in three transcript variants differing in the 5′ UTR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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