Thermo Fisher Scientific Protein C Polyclonal Antibody
Human Protein C에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 시약이며, 1 mg/mL 농도로 제공. HepG2 및 HCT116을 양성 대조군으로 권장하며 연구용으로만 사용.
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Thermo Fisher Scientific Protein C Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–236 of Human Protein C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2545797 |
Product Specific Information
- Recommended positive controls: HepG2, HCT116
- Predicted reactivity: Dog (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein C is a vitamin K-dependent plasma glycoprotein that is cleaved to its activated form by the thrombin-thrombomodulin complex. Activated Protein C contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII.
Protein C is produced in the liver and consists of two polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor, and the active enzyme (55 kDa) cleaves factor Va and VIIIa, inhibiting blood coagulation.
Normal concentrations in human plasma are approximately 1–3 ng/mL for the active enzyme and 3 µg/mL for the proenzyme. Mutations in the Protein C gene are associated with thrombophilia, neonatal purpura fulminans, and recurrent venous thrombosis.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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