Takara SP6 RNA Polymerase

Takara SP6 RNA Polymerase

Applications

• In vitro transcription

Supplied Buffer

10X Reaction Buffer 구성:
| 구성 성분 | 농도 |
|------------|------|
| Tris-HCl (pH 7.5) | 400 mM |
| MgCl₂ | 60 mM |
| Spermidine | 20 mM |
| DTT | 100 mM |
| BSA | 0.1% |

Product Citations

• Butler, E. T. & Chamberlins, M. J. Bacteriophage SP6-specific RNA Polymerase I. Isolation and characterization of the enzyme. J. Biol. Chem. 257, 5772–5778 (1982).
• Green, M. R., Maniatis, T. & Melton, D. A. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei. Cell 32, 681–694 (1983).
• Kotaoi, H., Ishizalci, Y., Hiraoka, N. & Obayashi, A. Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 15 (1987).
• Melton, D. A. et al. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035–7056 (1984).
• Pelletier, J. & Sonenberg, N. Insertion mutagenesis to increase secondary structure within the 5′ noncoding region of a eukaryotic mRNA reduces translational efficiency. Cell 40, 515–526 (1985).
• Sagawa, H., Ohshima, A. & Kato, I. A tightly regulated expression system in Escherichia coli with SP6 RNA polymerase. Gene 168 (1996).
• Schenborn, E. T. & Mierendorf, R. C. A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure. Nucleic Acids Res. 13, 6223–6236 (1985).

Additional Product Information

제품의 보관 조건, 구성 성분 및 기술 사양은 Certificate of Analysis에서 확인할 수 있습니다.
Kit 구성품 목록은 Documents 탭에서 확인 가능합니다.

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