Thermo Fisher Scientific c-Abl Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1130 of human c-Abl (NP_0051482) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Abl,uniProtId:P00519-1,ncbiNodeId:9606,antigenRange:1000-1130,antigenLength:1130,antigenImageFileName:PA5-87407_c-Abl_P00519-1_Rabbit.svg,antigenImageFileNamePDP:PA5-87407_c-Abl_P00519-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.34 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804126

Product Specific Information

Positive Samples: HeLa; Cellular Location: Cytoplasm, Lipid-anchor, Mitochondrion, Nucleus, Nucleus membrane, cytoskeleton

Immunogen sequence: TAFIPLISTR VSLRKTRQPP ERIASGAITK GVVLDSTEAL CLAISRNSEQ MASHSAVLEA GKNLYTFCVS YVDSIQQMRN KFAFREAINK LENNLRELQI CPATAGSGPA ATQDFSKLLS SVKEISDIVQ R

Target Information

The ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns c-Abl into an oncogene. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for c-Abl. In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome). As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The c-Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). c-ABL potentially regulates DNA repair by activating the proapoptotic pathway when the DNA damage is too severe to be repaired.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.