Thermo Fisher Scientific CD43 Recombinant Mouse Monoclonal Antibody (rSPN, 1094)
CD43 단백질을 표적으로 하는 마우스 유래 재조합 단클론 항체로, Western blot, IHC, ICC, 유세포분석 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며, Protein A/G로 정제되어 높은 순도를 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rSPN, 1094 |
| Immunogen | Recombinant full-length human CD43 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on the surface of most hematopoietic cells.
It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation.
Through interactions with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte trafficking.
CD43 signaling modulates LFA-1 adhesiveness and influences tumor suppressor p53 expression.
Dysfunction of CD43 is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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