Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), Brilliant Ultra Violet 805, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Species Reactivity

• Dog
• Human

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• eFluor 570
• eFluor 615
• eFluor 660
• FITC
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human and canine Ki-67 protein, which exists in two isoforms (345 and 395 kDa) expressed in dividing cells. Ki-67 is present during the active phases of the cell cycle (G1, S, G2, and mitosis) but absent in resting cells (G0). It localizes to the nucleus during interphase and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.

Ki-67 expression serves as a proliferation marker in tumor cell studies, indicating the fraction of proliferating cells within a tumor population.

Applications Reported

• Intracellular staining followed by flow cytometric analysis.

Applications Tested

• Pre-diluted and tested using intracellular staining of stimulated human peripheral blood cells with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
• Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information

• Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is designed for cytometers with a 355 nm UV laser.
• Use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) when combining multiple polymer dye-conjugated antibodies.
• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark.
• Methanol compatibility: Suitable for short-term methanol-based fixation (≤1 hour).

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and shows cell cycle–dependent localization. Ki-67 is widely used as a cell proliferation marker, as it is absent in G0 cells but increases through mitosis.
In tumor diagnostics, the Ki-67 index correlates strongly with tumor grade and serves as a reliable indicator of proliferative activity. It is also used as a neuronal marker for cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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