Thermo Fisher Scientific Chitotriosidase Polyclonal Antibody
마우스 Chitotriosidase 단백질을 인식하는 Sheep 유래 Polyclonal 항체. Western blot과 Immunoprecipitation에 적합. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. Gaucher 병 연구 등 macrophage 활성화 관련 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Chitotriosidase/CHIT1 (Ala22–Ser464) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2605584 |
Product Specific Information
- In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human CHIT1 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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