Thermo Fisher Scientific Human ADRBK1 (GRK2), His Tag Recombinant Protein
인간 ADRBK1(GRK2) 히스태그 재조합 단백질로, 세린/트레오닌 키나아제 활성을 연구하는 데 사용됩니다. 곤충 세포에서 발현된 정제된 액상 단백질이며, -80°C에서 안정적으로 보관 가능합니다. GPCR 신호전달 및 키나아제 조절 연구에 적합합니다.
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Thermo Fisher Scientific Human ADRBK1 (GRK2), His Tag Recombinant Protein
Applications
- Kinase Assay (KA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Insect cells |
| Amino Acid Sequence | Full length |
| Tag | His-tag |
| Molecular Weight | 82.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris HCl, pH 7.5, with 0.05% Triton X-100, 4 mM DTT, 0.5 mM EDTA, 100 mM NaCl, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- For maximum recovery, spin prior to use.
- Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
- For larger sizes, reduce freeze/thaw cycles by preparing aliquots (≥20 µL).
- Do not store diluted kinase.
- Properly stored at -80°C, guaranteed for 6 months from date of purchase.
- Protein Form: Full Length
Target Information
ADRBK1 (GRK2) is a serine/threonine kinase that phosphorylates G protein-coupled receptors (GPCRs). GRK2 (83 kDa) is ubiquitously expressed in mammals and plays a key role in regulating GPCR signaling. Upon ligand binding, GPCRs are phosphorylated by GRKs, leading to internalization mediated by beta-arrestin-1 and activation of Ras-Raf-ERK1/2 pathways. GRK2 activity is regulated by phosphorylation (PKC, Src, ERK1/2) and protein interactions. ERK phosphorylates GRK2 on serine 670, providing negative feedback regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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