Thermo Fisher Scientific SMURF2 Polyclonal Antibody
SMURF2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody입니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 사용 가능하며, Human, Mouse, Rat에서 반응합니다. Protein A로 정제된 고품질 항체로 연구용으로 적합합니다.
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Thermo Fisher Scientific SMURF2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human SMURF2 (amino acids 601–700) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 conjugating enzyme to targeted substrates. It interacts with SMAD1, SMAD2, and SMAD7 to trigger ubiquitination and proteasome-dependent degradation. SMURF2 enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression with SMAD1 leads to a significant decrease in SMAD1 protein levels and a smaller decrease in SMAD2 levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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