Thermo Fisher Scientific AP2 alpha Polyclonal Antibody
Western blot, IHC, ICC/IF에 사용 가능한 다목적 AP2 alpha 폴리클로날 항체. 인간 TFAP2A 단백질을 면역원으로 제작. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 4°C 또는 -20°C에서 안정 저장 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TFAP2A. Recombinant protein control fragment (Product #RP-103276). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791469 |
Product Specific Information
Immunogen sequence: GQRQSQESGL LHTHRGLPHQ LSGLDP
Target Information
TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in key biological functions such as eye, face, body wall, limb, and neural tube development. They also suppress genes including MCAM/MUC18, C/EBP alpha, and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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