Thermo Fisher Scientific Fibronectin Polyclonal Antibody
Fibronectin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. Affinity chromatography로 정제되었으며, PBS + 50% glycerol buffer에 보관. 세포 부착 및 이동 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human FN1 (Accession P02751), corresponding to amino acid residues Q2352–E2386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818245 |
Product Specific Information
This antibody detects endogenous levels of total Fibronectin.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight of 230–250 kDa. In connective tissues and blood vessels, fibronectin exists as an insoluble, cross-linked protein forming high molecular mass polymers. It is abundant during embryonic development and tissue remodeling and is also found as a soluble plasma protein.
Fibronectin functions in cell adhesion, migration, wound healing, blood coagulation, host defense, and metastasis. It is implicated in carcinoma development, particularly in non-small cell lung carcinoma, where its expression is elevated. Fibronectin facilitates tumor cell adhesion and confers resistance to apoptosis induced by chemotherapeutic agents.
The FN1 gene undergoes alternative splicing in three regions, potentially generating up to 20 transcript variants, although some full-length variants remain uncharacterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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