Thermo Fisher Scientific Phospho-Syk (Tyr525, Tyr526) Monoclonal Antibody (F.724.5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:100-1:400

-

Flow Cytometry (Flow)

1:200-1:800

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.724.5

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr525/526 of human Syk if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Syk,uniProtId:P43405-1,ncbiNodeId:9606,antigenRange:526,antigenLength:635,antigenImageFileName:MA5-14918_Syk_P43405-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14918_Syk_P43405-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

90 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10989513

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other tyrosine-phosphorylated protein tyrosine kinases.

Target Information

Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.