Thermo Fisher Scientific Human CSNK1E (CK1 epsilon) [R178C], GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

GST-tag

Molecular weight

73.6 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 0.25mM DTT, 0.1mM EDTA, 150mM NaCl, 10mM glutathione, 25% glycerol, 0.1mM PMSF

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This CSNK1E (CK1 epsilon) R178C recombinant human protein (full length) was expressed in insect cells.

CSNK1E (CK1 epsilon) R178C recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Z`-LYTE™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active

The gene accession number for CSNK1E (CK1 epsilon) [R178C] is NP_001885.1.

Target Information

CSNK1E serine/threonine kinase is a member of the CK1 family which plays an important role in diverse cell processes, including DNA replication and repair. CSNK1E is a regulator of Yes-associated protein (YAP) transcription coactivator, which is a key regulator of organ size and a candidate human oncogene. CSNK1E is activated by CCK2R and then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CSNK1E can phosphorylate topoisomerase (topo) II alpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.