Thermo Fisher Scientific IL-4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Neutralization (Neu)

2-8 µg/mL

-

Product Specifications

Species Reactivity

Dog

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609990

Product Specific Information

In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-4 is observed, 15% cross-reactivity with recombinant feline IL-4 and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant cotton rat IL-4, recombinant mouse IL-4, and recombinant rat IL-4 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.