Thermo Fisher Scientific APAF1 Monoclonal Antibody (2E10)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25-0.5 µg/mL

-

Immunohistochemistry (IHC)

0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E10

Immunogen

A peptide corresponding to amino acids near the carboxy terminus of human Apaf1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:APAF1,uniProtId:O14727-1,ncbiNodeId:9606,antigenRange:1248,antigenLength:1248,antigenImageFileName:MA5-15743_APAF1_O14727-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15743_APAF1_O14727-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154527

Product Specific Information

In Western blot applications, this antibody detects a band at ~130kDa. A suggested positive control is K562 cell lysate.

Target Information

APAF1 gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.