Thermo Fisher Scientific CD22 Polyclonal Antibody
Human CD22 단백질에 특이적인 Rabbit Polyclonal Antibody로, IHC(P)에서 1:1,000~1:2,500 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol 버퍼에 보존. B세포 표지자 연구 및 백혈병, 림프종 관련 연구에 적합.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:1,000–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CD22 (Product #RP-88562) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2856312 |
Product Specific Information
Immunogen sequence:
DLESFILFHN PEYNKNTSKF DGTRLYESTK DGKVPSEQKR VQFLGDKNKN CTLSIHPVHL NDSGQLGLRM ESKTEKWMER IHLNVSERPF PPHIQLPPEI QESQEVTLTC LLNFSCYGYP IQLQWLL
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein (130–140 kDa) expressed in the cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation, similar to CD19. CD22 membrane expression is limited to mature B cells (CD22+) and plasma cells (CD22−), useful for phenotyping mature leukemias. Strong expression is observed in hairy cell leukemia and diffuse large B-cell lymphoma, while it is negative in classical Hodgkin’s lymphoma.
CD22 preferentially binds α2,6-linked sialic acid, and its recognition site may be masked by cis interactions. Upon ligand-induced tyrosine phosphorylation, CD22 regulates B-cell receptor signaling, interacting with Src family kinases and recruiting phosphatases that modulate signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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