Thermo Fisher Scientific AMPK alpha-1 Polyclonal Antibody
AMPK alpha-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 액상 형태로 제공됩니다. 세포 내 에너지 대사 조절 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 381–520 of human AMPK alpha-1 (NP_0062425) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.89 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804932 |
Product Specific Information
Immunogen sequence:
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID
Positive Samples: HT-29, HeLa, HepG2, U-251MG, Mouse spleen, Mouse lung, Mouse kidney, Rat heart
Cellular Location: Cytoplasm, Nucleus
Target Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the γ phosphate of ATP, onto an acceptor amino acid in a substrate protein. Through this mechanism, they mediate most signal transduction in eukaryotic cells, regulating metabolism, transcription, cell cycle, cytoskeletal rearrangement, movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest in eukaryotes, classified into 8 major groups based on catalytic domain sequence comparisons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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