Thermo Fisher Scientific TCF12 Monoclonal Antibody (OTI4A7), TrueMAB
TCF12 단백질을 인식하는 단일클론 항체로, Western Blot 및 IHC(Paraffin) 분석에 적합합니다. Human, Mouse, Rat에 반응하며, IgG2a 아이소타입을 가진 Mouse 유래 항체입니다. 동결건조 형태로 제공되며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific TCF12 Monoclonal Antibody (OTI4A7), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A7 |
| Immunogen | Full-length human recombinant protein of human TCF12 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
TCF12 (also known as HTF4) is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the E-box consensus sequence (CANNTG).
It is expressed in various tissues including skeletal muscle, thymus, and lymphocytes (B- and T-cells).
TCF12 forms heterodimers with other bHLH E-proteins to regulate lineage-specific gene expression.
Together with E2A, TCF12 is essential for blocking thymocyte proliferation before pre-TCR expression and is critical for proper T cell differentiation.
Recent studies indicate that TCF12 also plays a key role in invariant natural killer T cell development.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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