Thermo Fisher Scientific RELM alpha Monoclonal Antibody (226033)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

8-25 µg/mL

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

226033

Immunogen

E. coli-derived recombinant mouse RELM alpha Asp24-Ser111 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RELM alpha,uniProtId:Q9EP95-1,ncbiNodeId:10090,antigenRange:24-111,antigenLength:111,antigenImageFileName:MA5-23898_RELM_alpha_Q9EP95-1_Rat.svg,antigenImageFileNamePDP:MA5-23898_RELM_alpha_Q9EP95-1_Rat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2605577

Product Specific Information

In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) RELM beta or rmResistin.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.