Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12,K16) Polyclonal Antibody
Histone H4의 K5, K8, K12, K16 아세틸화 형태를 인식하는 rabbit polyclonal antibody. Western blot, IHC, ICC, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ChIP assay (ChIP) | - | 1 publication |
| Dot blot (DB) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–103 of Human Histone H4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549502 |
Product Specific Information
- Recommended positive controls: HeLa, NIH-3T3, BCL-1, PC-12, TGW
- Predicted reactivity: Mouse (100%), Xenopus laevis (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules each of H2A, H2B, H3, and H4 form an octamer, around which approximately 146 bp of DNA is wrapped to form nucleosomes. The linker histone H1 binds linker DNA between nucleosomes and aids in chromatin compaction.
This gene encodes a member of the histone H4 family, is intronless, and located on chromosome 6. Transcripts lack polyA tails but contain a palindromic termination element.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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