Thermo Fisher Scientific YBX1/Y-box Binding Protein 1/YB-1 Monoclonal Antibody (YBX1, 2430)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed):

• Incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection.
This protein stabilizes cytoplasmic mRNA and modulates translation by interacting with eukaryotic initiation factors.
It binds to promoters containing a Y-box sequence (5'-CTGATTGGCCAA-3'), such as HLA class II genes, and regulates transcription of numerous genes.
YBX1 promotes separation of mismatched or cisplatin-modified DNA strands, exhibits endonucleolytic activity introducing nicks or breaks in double-stranded DNA (in vitro), and may play a role in DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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