Thermo Fisher Scientific BCKDHA Polyclonal Antibody
Thermo Fisher Scientific의 BCKDHA Polyclonal Antibody는 인간 BCKDHA 단백질을 인식하는 토끼 유래 IgG 항체로, WB, IHC, ICC/IF에 사용 가능합니다. 고순도 항원 친화 크로마토그래피로 정제되었으며, 다양한 종에서 높은 예측 반응성을 보입니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 252–445 of Human BCKDHA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.31 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546776 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2
- Predicted reactivity: Mouse (95%), Rat (95%), Pig (94%), Sheep (95%), Chimpanzee (98%), Bovine (94%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of branched-chain amino acids.
The BCKD complex consists of three catalytic components:
- A heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase (E2)
- A homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also includes two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일: PA5-29300_BCKDHA_P12694-1_Rabbit.svg, PA5-29300_BCKDHA_P12694-1_Rabbit_PDP.jpeg)
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