Thermo Fisher Scientific MTDH Monoclonal Antibody (OTI1H1), TrueMAB
MTDH 단백질을 인식하는 Mouse monoclonal antibody로, Western blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, Lyophilized 형태로 제공. Metadherin 단백질 연구 및 암 전이 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 70–582 of human MTDH produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Metadherin (MTDH, also known as LYRIC) is a novel protein associated with tight junction proteins ZO-1 and occludin in polarized epithelial cells.
It is not a structural component but is recruited during tight junction maturation.
Metadherin is overexpressed in breast cancer tissues and xenografts, with lower expression in normal breast tissue.
It binds to lung vasculature via a C-terminal extracellular domain segment; blocking this domain or inhibiting metadherin expression can reduce breast cancer metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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