Thermo Fisher Scientific CD153 Monoclonal Antibody (RM153), eBioscience

Thermo Fisher Scientific CD153 Monoclonal Antibody (RM153), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• PE Conjugate
• Request custom conjugation

Product Specific Information

Description:
The RM153 monoclonal antibody reacts with mouse CD153 (CD30 ligand), a 40 kDa type II transmembrane protein belonging to the TNF superfamily. Interaction of CD30 with CD153 is critical in T cell activation and costimulation. CD153 expression on T cells is upregulated via TCR engagement and peaks 24–48 hours after activation. Signaling through CD153 is important for costimulation of pre-activated T cells.

Applications Reported:
Reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Also used in blocking ligand binding (Functional Grade purified RM153, Product #16-1531 recommended for functional assays).

Applications Tested:
Tested by flow cytometric analysis of activated mouse splenocytes. Recommended use ≤0.5 µg per test (100 µL final volume). Optimal cell number and antibody concentration should be empirically determined.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD30 ligand (CD30L/CD153) binding mediates diverse cellular responses including proliferation, activation, differentiation, and apoptosis. CD30 plays a key role in Hodgkin’s disease and CD30⁺ lymphomas, and acts as a costimulatory molecule in thymic negative selection. CD30 is expressed on Hodgkin’s and Reed–Sternberg cells, some non-Hodgkin’s lymphomas (e.g., Burkitt’s), virus-infected T/B cells, and activated normal T/B cells. In T cells, CD30 is found on subsets producing Th2-type cytokines and on CD4⁺/CD8⁺ thymocytes coexpressing CD45RO and IL4 receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.