Thermo Fisher Scientific Perilipin A/B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Perilipin A/B,uniProtId:P43884-1,ncbiNodeId:10114,antigenRange:1-17,antigenLength:517,antigenImageFileName:PA1-1052_Perilipin_AB_P43884-1_Rabbit.svg,antigenImageFileNamePDP:PA1-1052_Perilipin_AB_P43884-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2268558

Product Specific Information

PA1-1052 detects perilipin A and B from rat and 3T3-L1 cell extract.

PA1-1052 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~62 and 46 kDa proteins representing perilipin A and B, respectively from 3T3-L1 cell extract

The PA1-1052 immunogen is a synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B. This sequence is 82% conserved in humans. The PA1-1052 immunizing peptide (Cat. # PEP-170) is available for use in neutralization and control experiments.

Target Information

Adipose tissue is an energy reserve in animals and is strictly regulated in nondomestic species. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), resistin, and perilipin. Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.