Thermo Fisher Scientific Perilipin A/B Polyclonal Antibody
Thermo Fisher Scientific의 Perilipin A/B Polyclonal Antibody는 Rat 및 3T3-L1 세포에서 Perilipin A와 B 단백질을 검출합니다. Western blot에 최적화되어 있으며, 62 kDa 및 46 kDa 단백질 밴드를 인식합니다. PBS 기반 용액 형태로 제공되며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 6 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues M(1) S M N K G P T L L D G D L P E Q(17) C of rat Perilipin A and B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2268558 |
Product Specific Information
- PA1-1052 detects Perilipin A and B from rat and 3T3-L1 cell extract.
- Successfully used in Western blot procedures.
- Detects approximately 62 kDa (Perilipin A) and 46 kDa (Perilipin B) proteins from 3T3-L1 cell extract.
- Immunogen sequence is 82% conserved in humans.
- The immunizing peptide (Cat. # PEP-170) is available for neutralization and control experiments.
Target Information
Adipose tissue serves as an energy reserve and is tightly regulated. Adipocytes secrete key proteins such as lipoprotein lipase (LPL), leptin, Acrp30, resistin, and perilipin.
Perilipin is a hormonally regulated intracellular lipid droplet protein localized to lipid droplet surfaces.
Upon lipolytic stimulation, perilipin is phosphorylated by protein kinase A and modulates hormone-sensitive lipase (HSL) activity, influencing triacylglycerol hydrolysis.
Perilipin expression is restricted to adipocytes and steroidogenic cells, existing as two isoforms (A and B) derived from a single gene via alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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