Thermo Fisher Scientific His-tag Polyclonal Antibody

Applications and Tested Dilution

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Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
His-tag Antibody, pAb, Rabbit recognizes His-tagged proteins in transfected or transformed cells.
Short-sequence epitope tags are commonly used in protein expression studies across various expression systems.
The His tag enables efficient coupling to Ni²⁺ affinity resins and purification by single-step chromatography.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is typically expressed at either the N- or C-terminal of recombinant proteins to facilitate isolation or purification via immobilized metal affinity chromatography (IMAC).

Epitope tagging fuses a known epitope to a recombinant protein, allowing detection even when no specific antibody exists.
His-tagged proteins containing histidine clusters can be purified through metal chelate chromatography due to the metal-binding properties of histidine residues.
The affinity between histidine and Ni²⁺ ions enables strong, selective purification using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are valuable tools for detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.