Thermo Fisher Scientific MBP-tag Monoclonal Antibody (5B10B11)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

ELISA (ELISA)

0.1-1.0 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B10B11

Immunogen

Purified recombinant MBP fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP tagged protein is purified with an amylose column. The MBP tagged protein can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein. MBP-tag Antibody, mAb, Mouse is suitable for detecting fusion proteins that contain a MBP-tag.GenScript MBP-tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant MBP fusion protein.

Target Information

The approach of epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP-tagged product. Cleavage by factor Xa separates MBP from its partner protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.