Thermo Fisher Scientific HGF Monoclonal Antibody (OTI1D2), TrueMAB
인간 HGF 단백질을 인식하는 마우스 단클론 항체로 Western blot 및 IHC(P) 적용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 세포 성장 및 형태 형성 연구용으로 적합. 연구용 전용 제품.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 200–494 of human HGF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Hepatocyte Growth Factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade through binding to the proto-oncogenic c-Met receptor.
Secreted by mesenchymal cells, HGF acts as a multifunctional cytokine on epithelial cells, promoting mitogenesis, motility, and matrix invasion, which are essential in angiogenesis, tumorogenesis, and tissue regeneration.
It is secreted as a single inactive polypeptide and cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain, linked by a disulfide bond to form the active heterodimer.
Although belonging to the plasminogen subfamily of S1 peptidases, HGF has no detectable protease activity.
Alternative splicing generates multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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