Thermo Fisher Scientific NF-M Polyclonal Antibody
Rabbit polyclonal antibody against NF-M for detection in WB, IHC, ICC, Flow, and ELISA. Reacts with chicken, human, mouse, and rat. Protein A purified, 1 mg/mL in TBS with BSA and glycerol. Suitable for neuronal cytoskeletal research.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from rat NF-M, amino acids 101–200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Neurofilaments (NFs) are intermediate filaments expressed almost exclusively in neuronal cells, especially in large axons. They consist of three major subunits—NF-M (medium), NF-H (high), and NF-L (light)—which share a coiled-coil core domain but differ in terminal regions. The NF-M protein tail plays a key role in axonal size and cytoskeletal organization through phosphorylation-dependent interactions. NF-M phosphorylation and O-GlcNAcylation are reciprocally regulated, influencing filament formation and function. Antibodies against neurofilament subunits serve as valuable markers for neuronal cells due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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