Thermo Fisher Scientific Papain Polyclonal Antibody
Papain 단백질을 인식하는 염소 유래 다클론 항체로, WB, IHC, ELISA, IP 등 다양한 응용에 적합함. 고순도 이온교환 크로마토그래피로 정제되었으며, 식물성 단백질 반응성 확인됨. 장기 보관 시 -20°C에서 안정적으로 유지 가능.
- 카탈로그번호
- 200-1174xxxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Papain Polyclonal Antibody
Thermo Fisher Scientific Papain Polyclonal Antibody
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:3,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Plant |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Papain Antibody was produced by repeated immunizations with pork Papain protein isolated from papaya. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Papain antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, ion-exchange chromatography, and extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Papain (Carica papaya). Cross-reactivity against Papain from other sources may occur but has not been specifically determined.
Target Information
Papain is a protease derived from the papaya fruit (Carica papaya). It hydrolyzes peptides with broad specificity but shows a preference for large hydrophobic amino acids in the P2 position. It is synthesized as an inactive 40 kDa precursor containing a 107-residue N-terminal pro region and a 26-residue signal peptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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