Thermo Fisher Scientific Lactate Dehydrogenase Polyclonal Antibody
Rabbit muscle 유래 LDH 단백질로부터 제작된 Goat polyclonal antibody로, WB, ICC/IF, ELISA, IP 등에 사용 가능. 고순도 IgG로 정제되어 안정성이 높으며, 대부분의 포유류 LDH-A/B 아이소폼과 교차반응. 연구용으로만 사용.
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Thermo Fisher Scientific Lactate Dehydrogenase Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Prepared from whole goat serum immunized with full-length lactate dehydrogenase protein isolated from rabbit muscle |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid; dilute only prior to immediate use.
Anti-Lactate Dehydrogenase antibody is an IgG fraction purified from monospecific antiserum via delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Immunoelectrophoresis showed a single precipitin arc against anti-Goat Serum and purified Lactate Dehydrogenase (Rabbit Muscle).
BLAST analysis suggests cross-reactivity with muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Target Information
Lactate dehydrogenase (LDH) catalyzes the conversion of pyruvate to lactate with interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes depending on the M/H chain composition in the tetrameric structure.
LDHA expression is hypoxia-inducible and regulated by HIF1α activity.
LDHB is upregulated in various cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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