Thermo Fisher Scientific ERCC1 Monoclonal Antibody (2E12), UltraMAB
ERCC1 단백질을 인식하는 Mouse 단일클론 항체로, Western blot, IHC, ICC, ChIP 등 다양한 응용에 사용 가능. Human 및 Rat 시료에 반응하며, 동결건조 형태로 제공. DNA 손상 복구 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ChIP assay (ChIP) | Assay-dependent | |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E12 |
| Immunogen | Full length human recombinant ERCC1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5′-incision during DNA repair.
It functions with SLX4 in the repair of interstrand cross-links (ICL) and participates in processing anaphase bridge-generating DNA structures.
ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks in conjunction with SLX4.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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