Thermo Fisher Scientific ERCC1 Monoclonal Antibody (4F9), UltraMAB
ERCC1 단백질을 인식하는 4F9 클론의 Mouse Monoclonal 항체로, Western blot, IHC, ICC, ChIP 등 다양한 응용에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. DNA 복구 연구 및 단백질 상호작용 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ChIP assay (ChIP) | Assay-Dependent | |
| Peptide Array (Array) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F9 |
| Immunogen | Protein expressed in 293T cell transfected with human ERCC1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ERCC1 is a non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Together with SLX4, it mediates the initial step in the repair of interstrand cross-links (ICL) and participates in processing anaphase bridge-generating DNA structures that may cause cytokinesis failure.
ERCC1 is also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
(Source: UniProt)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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