Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1.0 µg/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells.

Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions. Commonly used as a phenotypic marker for mouse T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤1.0 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirical optimization recommended).

Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737) — emits at 732 nm; use with UV (355 nm) laser-equipped cytometers.

Buffer Recommendations:

• Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)

Methanol Compatibility: Short-term fixation compatible; avoid long-term methanol exposure (>1 hour).

Excitation / Emission / Laser: 355 nm / 732 nm / Ultraviolet Laser

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company; Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits encoded on chromosome 11. These subunits contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction.

CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Abnormal CD3 expression may occur in autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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