Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/Test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It exhibits immunosuppressive properties and has been used in organ transplant rejection treatments. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and expression, and CD3 is expressed by thymocytes and all mature T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in 100 µL final volume.

Brilliant Ultra Violet™ 737 (BUV737):

• Tandem dye emitting at 732 nm
• Compatible with UV (355 nm) laser-equipped cytometers
• Sensitive to photo-induced oxidation (protect from light)

When using polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
BUV737 is compatible with short-term methanol fixation but should not be stored in methanol-containing buffers for more than one hour.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (γ, δ, ε, ζ) are immunoglobulin superfamily members encoded on chromosome 11. They interact with signal transduction molecules via tyrosine-based motifs. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

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