Thermo Fisher Scientific GLI1 Monoclonal Antibody (OTI1E5), TrueMAB
GLI1 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 최적화됨. 인간 시료에 반응하며, 동결건조 형태로 제공. PBS 버퍼(8% 트레할로스) 내 보관, -20°C에서 안정적 저장 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
- Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 755–1106 of human GLI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the Sonic Hedgehog signal transduction cascade and regulates stem cell proliferation. Its activity and nuclear localization are negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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