Thermo Fisher Scientific GRK2 Polyclonal Antibody
GRK2 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체. Western blot에 최적화되어 있으며, 다양한 종에서 교차 반응 예측. 0.5 mg/mL 농도의 액상 형태로 제공되며, 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 0.3–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (RDAYREAQQLVQR) corresponding to internal amino acids of ADRBK1 (aa 648–661) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10989423 |
Product Specific Information
- Predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
ADRBK1 (GRK2) is a serine/threonine kinase that phosphorylates G protein-coupled receptors (GPCRs). GRK2 (83 kDa) is ubiquitously expressed in mammals and plays a key role in regulating GPCR signaling. Upon ligand binding, GPCRs are phosphorylated by GRKs, leading to receptor internalization via beta-arrestin-1 and activation of the Ras–Raf–ERK1/2 pathway. GRK2 activity is regulated by phosphorylation (PKC, Src, ERK1/2) and protein interactions. ERK-mediated phosphorylation at serine 670 inactivates GRK2 in a feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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