Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Functional Grade, eBioscience
인간 및 마우스 CD80(B7-1)을 인식하는 2D10.4 단클론 항체로, Flow Cytometry 및 Functional Assay에 적합합니다. 활성화된 B세포, 대식세포, 수지상세포에서 발현되는 CD80을 검출하며, 고순도(>90%)와 저엔도톡신 수준(<0.001 ng/µg)을 제공합니다. 연구용으로만 사용됩니다.
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- 16-0809-xxx (3개 옵션)
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3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Functional Grade, eBioscience
Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (2D10.4), Functional Grade, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 13 publications |
| Functional Assay | Assay-Dependent | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D10.4 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2865798 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 711
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages, and dendritic cells, as well as activated T cells. It binds with high affinity to CD28 and CD152 (CTLA-4) on T cells, playing a crucial role in T-B cell communication and activation.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage ≤1 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg (LAL assay).
Aggregation: <10% (HPLC).
Target Information
CD80 (B7-1) and CD86 (B7-2) act as ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on professional antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cells, follicular B lymphoma cells, T cells, and monocytes. Dysfunction of CD80 has been linked to gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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