Thermo Fisher Scientific BPI Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total BPI.

Target Information

The antimicrobial protein BPI is a 55 kDa protein found in the primary (azurophilic) granules of human neutrophils and also detected on the surface of neutrophils, small intestinal, and oral epithelial cells.
BPI is a bactericidal compound present in polymorphonuclear cells (PMN) and in lower levels in eosinophils.
It binds with high affinity to the lipid A region of lipopolysaccharides (LPS) from gram-negative bacteria, leading to:

• Cytotoxicity via sequential damage to bacterial membranes
• Neutralization of gram-negative bacterial LPS
• Opsonization to enhance phagocytosis by neutrophils

BPI may play a role in LPS-triggered airway disease development, as airway epithelial cells express and produce BPI.
Inflammation induced by LPS may contribute to rapid airflow decline, a serious complication in hematopoietic cell transplantation.
A 21 kDa recombinant fragment of BPI, rBPI21, has shown survival advantage against invasive pneumococcal disease by binding to pneumolysin from gram-positive bacteria.

For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.